Research Of High Yield Technology And Strains Selection Of Cordyceps Militarris

This study focused on the fungi of Cordyceps militaris, a famous traditional Chinese edible and medicinal mushroom. We collected 12 wild Cordyceps militaris. The optimal culture requirements for mycelial and fruitbody growth were investigated. In order to established an integrated method of good strains selection, the biological character, the fermentation cultures and the content of the primary three active component (polysaccharide, cordycepin and mannitol) were studied. We obtained the primary conclusions as follow:1, There were big difference in shape and yield between wild and cultivated fruitbody of Cordyceps militaris. The perithecium was ampullaceous. The ascus was linear and slightness in perithecia. There were two forms to produce spores.2, The research showed that the optimal culture requirements for mycelial growth was self-made cultured medium, the optimal carbon and nitrogen were glucose and peptone. The highest yield of mycelium was obtained from a 8-day culture grown in submerged cultures with an initial pH of 6.5, at 21 degrees C with shaking-induced aeration at 120 rpm. The yield of mycelium was high when put 1 circularity mycelium whose diameter was 6mm into 10ml cultured medium. KH₂PO₄(0.2%), MgSO₄ · 7H₂O(0.075%) and VtaminB(5mg/L) had significant effects on the growth of the mycelium.3, We found seven strains from 12 collected wild Cordyceps militaris whose yield of fruitbody was high. The contents of cordycepin were achieved by high-performance liquid chromatography (HPLC) using a Kromasil NH₂ analytical column with UV detector . The contents of amylase and mannitol were determined by Spectrophotometer. The contents of P, K, Fe, Z, Cu, Mn were determined too.At last, we came to the conclusion that S-4 and S-9 were the good strains with high yield of fruitbody and high content of active components.