Establishment And Clinical Application Of The Reference Program For The Measurement Of Catalytic Concentration Of Aspartate Aminotransferase And Aminotransferase(37℃)

Background and objectiveIt is very important to determine some concentration of enzymes in serum or other tissues for diagnosing and preventing from diseases. Measuring enzymes by two ways: one is determining qualities of enzymes by immunological methods, the other is determining catalytic concentration by clinical chemistry. The latter is often used, as it is rapid, sensitive and cheap. Catalytic concentration of enzymes is not only depending on content of enzymes, but also depending on many factors such as characters and concentration of substrates, pH of reaction medium, temperature, ion intension, activation or restrain factors. There are many reference ranges in the same enzyme so that the results in various labs can not be compared each other. Sometimes it will lead to clinical errors. Errors caused by enzymes concentration vary more than other small compounds in many clinical labs in our country. For better accuracy and precision, compare with results in different labs, eliminate errors in reference range and make for clinical application, we have to develop standardization of enzymes. For recent 20 years, standardization has been developed from three aspects: first: put forward to applying to reference methods; second: apply reference materials to calibrate routine methods; three: establishing nets of reference lab. Aspartate Aminotransferase(AST) and aminotransferase(ALT) are routine items used in clinical labs, so accuracy of them is very important for clinical results. Our objective is to establish the reference methods to measure catalytic concentration of AST and ALT at 37℃ according to IFCC primary procedure for the...