Study On Expression Of P1GF And Its Correlations With Angiogenesis In Ovarian Serous Cystadenoma

Background and Objective: Ovarian Serous Cystadenoma (OSC) is a common tumor of wemen,with the propotion of 25% in ovarian neoplasms.But its pathogenesis of OSC is unknown.Recent studies have showed that angiogenesis plays an important role in carcinogenesis and progression. Vascular endothelial growth factor(VEGF) is the most important factor of angiogenesis .Placenta growth factor(PlGF) is a new member of VEGF family, which has been recognized as the major growth factor in tumor angiogenesis,but there is not study on OSC.The aim of this study is to investigate the role of PlGF in the angiogenesis of this ovarian tumor,and to evaluate expression of PlGF in OSC and correlation with expression of VEGF and CD34 protein, and then provide a new indicator for early diagnosis and prognosis.Materials and Methods:90 cases of ovarian serous cystadenoma are from Centrat Hospital of Fushun, Oncological Hospital of Fushun and General Hospital of Shenyang Millitary Region(1986-2005).The age of patients is from 18 to 79.The sections are recut for 4um and restained with routine H.E.Immunohistochemistry:Streptavidin/Peroxidase(SP) method is used to detect the expression of PlGF,VEGF and CD34 in 90 cases of OSC.The positive immunostaining results of PlGF,VEGF and CD34 are clearly diffuse brown-yellowish granules both in plasma and membrane of the tumor cells.Microvessel- density (MVD) is determined by counting vessels in three high-power fields randomly selected (*400 magnification).The results are statistical analysis.Results:1.Positive expression of PlGF are observed in benign cystadenoma,borderline serous neoplasm and cystadeno- carcinoma,but the ratio of PlGF expression in carcinoma is significantly higher than the other...