VEGF, PLGF, P53 Expression In Hepatocellular Carcinoma And Angiogenesis Correlation

Objectiv:To investigate the correlation of the expression of vascular endothelial growth factor (VEGF), placental growth factor (PLGF) and p53 protein with primary hepatocellular carcinoma (HCC) and to study the probably mechanism of the co-expression of VEGF, PLGF, P53 and the occurrence of the HCC.Methods The expressions of VEGF, PLGF and the p53 protein in the 39 patients suffering from HCC were detected by Immunohistochemical SP and MVD were quantitated in HCC tissues,compared with clinicopathological parameters were assayed.Results:The expression rates of VEGF, PLGF, and p53 protein in the 39 patients suffering from HCC was 84.62% and 76.92% and 79.48% respectively; The expressions of VEGF protein was mainly found in the cytoplasm or membrane of tumor cells, there was significant differences qualitative in its expression diffused or concentrated in the cancer nest which closed to the margin or envelope of the tumor.The degree of VEGF expression was significantly high, but it had no correlation with tumor size, AFP and histological grade. The expressions of PLGF protein was mainly found in the cytoplasm or membrane of tumor cells, The expression rates of PLGF was significantly higher in the group with metastasis and recurrence than without metastasis and recurrence, There was no significant correlation between the positive expression of PLGF in hepatocellular carcinoma and tumor size and AFP. The expression rates of PLGF increased with the degree of pathological grade. The expressions of P53 protein in the nucleus showed brown, the colored cells showed mostly diffuse distribution, There was significant correlation between p53 protein and tumor grade (P<0.05), HBsAg and metastatic potential (P< 0.01), There was no significant correlation between p53 protein and the AFP and tumor size. HCC tissues by CD34 staining capillaries brown or brown, the positive staining confined to the cytoplasm and membrane of vascular endothelial form with diffuse distribution. The mean of MVD in 39 cases was 50.20±13.89. There was a significant difference in MVD expression and pathological grade, AFP, metastatic potential and HBsAg, the difference was statistically significant, but no significant difference in tumor size. There was correlation between the expressions of VEGF and PLGF in HCC (χ2= 7.59, P= 0.018, Table 3). MVD was 52.67±13.65 and 37.25±5.55 respectively, when the positive or negative expression of VEGF,the differences are significant (t=-3.124, P= 0.003); MVD was 51±13.77 and 40.36±7.60 respectively, when the positive or negative expression of PLGF the differences are significant (t= 2.462, P= 0.046). The co-expression of in liver tissue specimens showed that There was significant correlation between the expression of a variety of joint co-expression and differentiation factors, AFP, hepatitis B virus infection and metastatic potential. There was significant correlation between VEGF and p53 or between VEGF and PLGF P<0.01. After multivariate analysis, There was also significant correlation in VEGF, p53, PLGF, P＜0.01.Conclusion:There was overexpression of VEGF, PLGF, p53 protein in HCC tissue. The promotion of the growth and metastasis in HCC was made by promoting tumor angiogenesis. p53, PLGF and VEGF was responsible of an important indicator of biological behavior. HCC, The expression of VEGF, PLGF and p53 gene has significant correlation. The co-expression of VEGF, P53, PLGF in HCC has significant correlation with the degree of malignancy, metastasis and recurrence.