The Effects Of Semen On Endometrial Receptivity In Mice

Objective: To investigate the effect of semen on endometrial receptivity in the mouse by determing the expression of endometrial pinopodes, the level of endometrial interleukin-6 (IL-6) and implantation rate.Methods: Kunming female mice were randomly assigned to three groups: semen, seminal plasma and control group. The semen group received bilateral tubal ligation, then three weeks later after inducing ovulation mated with intact males, there is semen (seminal plasma and spermatozoa) in the uterus; the seminal plasma group after inducing ovulation mated with males three weeks after vasectomized, there is seminal plasma but no spermatozoa in the uterus; and the control group after inducing ovulation didn't cage with male mice, there is no semen in the uterus. The uterine speciments of three groups were taken respectively during day 2-6. The expression of endometrial pinopodes was determined by scanning electron microscopy and endometrial levels of IL-6 were measured by enzyme-linked immunosorbant assay (ELISA). Blastocysts were transferred to day 3 female mice of the three groups to compare the implantation rates.Results: 1.The expression of pinopodes in endometrial surface in the mice were obviously better in groups of seminal plasma and semen than in the control group, but there is no significantly difference between seminal plasma and semen group. 2.The changes of the level IL-6 in endometrium in three groups were similar, it increased obviously from day 3 and reached the peak on day 4 which was about twice of that of day...