A Preliminary Study Of Antitumor Immunity Induced By Exosomes Derived From Human Cord Blood Dendritic Cell Transfected With Tumor Cell Total RNA

Background and Objective:Dendritic cells (DC) are professional antigen presenting cells with the unique capacity to induce primary and secondary immune responses in vivo. It is reported that DCs secrete antigen presenting vesicles, called exosomes, which express functional Major Histocompatibility Complex class I and class II, and T-cell costimulatory molecules. Exosomes are membrane vesicles with a diameter of 30-100nm that are secreted by many cell types into the extracellular milieu,such as mast cells, B lymphocyte,T lymphocyte, blood platelet, tumor cell and DC.It is reported tumor peptide-pulsed DC-derived exosomes prime specific cytotoxic T lymphocytes in vivo and eradicate or suppress growth of established murine tumors in a T cell-dependent manner. Exosome-based cell-free vaccines represent an alternative to DC adoptive therapy for suppressing tumor growth.DC transfected with total RNA are also able to stimulate potent, T cell-mediated antitumor immune responses in vitro.Here,we use BGC-823 cell or K562 cell total RNA to transfect DC.To investigate whether exosomes derived from human cord blood derived dendritic cells transfected with tumor total RNA were...