Inhibition Of Survivin Gene Expression In Prostate Cancer Cell Line PC-3 By Vector-based RNAi

Prostate cancer, one of the most prevalent malignant tumors in older males, takes the first place for American old men on the list of male tumor incidence rate. The incidence rate has been increasing year by year with the change of average life span and dietary structure in China. Except operation, there is no optimal protocol to treat the prostatic carcinoma and it's very necessary to explore some new strategies for this malignant disease.Survivin encodes 142 arnino acids, 16.5KD intracellular protein that belongs to the inhibitor of apoptosis (IAP) gene family. Its chromosomal location is 17q25. Survivin is a bifunctional member of the inhibitor of apoptosis gene family that counteracts cell death and controls mitotic progression. Survivin that is present during embryonic development, is undetectable in most healthy adult tissues and becomes prominently overexpressed in nearly all human cancers. Predictive and prognostic detection of survivin has been implemented in some types of cancer. Two general considerations make survivin an attractive therapeutic target in cancer: it is selectively expressed in tumor cells and it is required for their viability.Since its formal discovery in 1998, RNA interference (RNAi) has rapidly...