Brain Injury And The Variation Of S-100B Protein And Therapeutic Effect By Rosiglitazone InRats In Acute Pancreatitis

Objective:To investigate pathological changes and the variation of S-100B protein and to explore the therapeutic effect of rosiglitanoze in early stage of acute pancreatitis-associated brain injury models in rats . At the same time to explain the mechanism of brain damage in early stage of acute pancreatitis-associated brain injury, and provide a new trial for clinical therapy and diagnoses in acute pancreatitis .Methods:Acute pancreatitis-associated brain injury was induced in rats by retrograde injection TCA into biliopancreatic ducts. At 3,6 and 12 hours after induction, S-100B protein were quantitated in serum, cerebrospinal fluid using ELISA kits. The mRNA for iNOS and PPARγin brain levels were measured by semi quantitative RT-PCR. The severity of pancreatitis was determined by serum amylase, and local pathologic lesions (gross and histopathologic scoring ), the brain injury were measured by tissue water content, iNOS activity, and histopathologic scoring. The effect of rosiglitanoze and the variation of S-100B protein were observed. Analysis of correlation among them were performed.Results :1,Brain injury occurred at 6 hours after induction in ANP. At 12 hours, seemed more serverely.2. S-100B protein in serum and cerebrospinal fluid increased at 6 hours after induction in severe acute pancreatitis and more at 12 hours after induction.3. S-100B protein level in serum and cerebrospinal fluid were positively correlated...