Experimental Study On Differentiation Of Bone Marrow Meseachymal Stem Cells Into Cardiomyocytes In Vitro And Transplantation Into Infracted Hearts

Objective: Myocardial infarction is the leading cause of heart failure in developed countries. The therapeutic measures of today are usually not sufficient to prevent left ventricular remodeling as they fall short of actual replacement of necrotic cardiac myocytes. Bone marrow mesenchymal stem cells ( MSCs ) are self-renewing, clonal precursors of non-hematopoietic tissues. They are expandable in culture and multipotent, and can differentiate into osteoblasts, chondrocytes, astrocytes, neurons and skeletal muscle. Implantation of bone-marrow stem cells in the heart might be a new method to restore tissue viability after myocardial infarction. Accordingly , we set out to isolate and expand a highly purified population of adult rat bone marrow-derived MSCs, and induce cardiomyocyte differentiation to therapy the infracted model.Method: (1) Isolate Sprague-Dawley (SD) rats' bone marrow mesenchymal stem cells( MSCs), and culture in vitro , observe their growth character and phenotype ; (2) Isolate and culture myocardial cells from neonate SD rat , observe their growth character and phenotype ; (3) Induce MSCs differentiate into myocardial cells and identify the results by immunofluorescence and RT-PCR ; (4) establish rat myocardial infarction model and identified by electrocardiogram, heart transonic and serum myocardium zymogram ; (5) Therapy the rat myocardial infaracted model with differentiated or undifferentiated MSCs , and estimate the therapeutic effect by cardiac functional parameter.Result: (1) MSCs divided from SD rat bone marrow Fusiform like and adherencegrowth , and can be expanded rapidly . The immunofluorescence and flow cytometer shows this group cells highly expressed CD54(ICAM-1 ), CD90( thy-1 )molecule and low express CD45molecule . (2) myocardium cells divided from neonate rat grow like...