Effects Of Intravenous Rutoside On Experimental AP And On TNF-α,IL-1β,IL-6,Pancreatic Cell Apoptosis And Its Mechanisms

Objective The purpose of this study is to investigate the effects of rutoside (Ru) intravenously on experimental aute pancreatitis (AP) in rats by retrograde injection of sodium taurocholate into biliopancreatic duct and in mice by intraperitoneal injections with caerulein plus lipopolysaccharide (LPS), and to study the effects of Ru on TNF-α, IL-1β, IL-6 of serum in rats with AP and on pancreatic acinar cell apoptosis and its moleculat mechanisms.Methods The AP model in rats was induced by retrograde injection of 3% sodium taurocholate (STC) into biliopancreatic duct. Ru (15, 30, 60 mg/(kg×h)) was administered by continuous venous infusion (CVI) for 6 hours immediately after the induction of AP. The AP in mouse model was induced by intraperitoneal injections with caerulein plus LPS. Ru (42, 84mg/kg) was administered by intravenous injection (i.v.) once an hour and a half for 4 times immediately after the induction of AP. The histopathological changes of pancreas were observed under light microscope and electronic microscopy. Serum amylase, the coefficient of pancreas , ascitic volume, amylase concentration in ascites in rats and the coefficient of pancreas, serum amylase and lactate dehydrogenase (LDH) in mice were determined. TNF-α, IL-1β and IL-6 of serum in rats were detected by radioimmunoassay. A terminal deoxynucleotidyl transferase-mediated dUTP-biotion nick end labeling(TUNEL) method was used to detect apotosis of pancreatic acinar cell and the expressions of NF-κB, Fas, FasL were...