The Expression Of MMP-9 & VEGF And It's Clinical Significance In Bladder Transitional Epithelial Cell Carcinoma

Objective: To study the expression and clinical significance of matrix metalloproteinase 9 (MMP-9) and vascular endothelial growth factor (VEGF) in bladder transitional epithelial cell carcinoma . Methods: Immunohistochemical S-P method was used to study the expression of MMP-9 and VEGF in 46 cases of bladder transitional epithelial cell carcinoma and 10 cases of normal bladder tissues . When making pathological tissue section , investment of paraffin was used , and respectively added mouse anti-human MMP-9 antibody and mouse anti-human VEGF antibody to incabate for 60 minutes at room temperature , then washed with PBS buffer 3 times . Again added biotin-conjugated goat anti-mouse antibody to incabate for 20 minutes at room temperature , and washed with PBS buffer 3 times . Once more add streptavidin-HRP to incabate , after washing with PBS buffer added DAB and H₂O₂ to colour the liquor . Then washed with water , and restained by using hematoxylin , dehydrated stepwise with alcohol and hyalinized with dimethylbenzene at last . Neutral gum was added and cover the slide glass , which could be observed under light microscope . Results: The expression rate of MMP-9 and VEGF in bladder carcinoma was 76.09 % and 67.39 % respectively , which was higher than that in normal group . (non-tumor bladder group) and significantly different from the normal group (p < 0.01). And the different expressions of MMP-9 and VEGF were related to comparing the well differentiated bladder cancer with the moderately and poorly differentiated bladder cancer (p < 0.05). Additionally , the expression level of MMP-9 and VEGF was different in the grade of Ta-T₁ and T₂-T₄ (p < 0.01). Conclusions: The positive expression of MMP-9 and VEGF in bladder transitional cell carcinoma tissue...