Expression And Significance Of Pituitary Tumor Transforming Gene-1,Matrix Metalloproteinase-2,Vascular Endothelial Growth Factor And Basic Fibroblast Growth Factor In Ovarian Serous Tumor

Objective:Pituitary tumor transforming gene (PTTG) is a novel oncogene that is expressed at higher level in most of the tumors analyzed to date compared to normal tissues. Existence of a relationship between PTTG1 levels and tumor malignant transformation, angiogenesis and metastasis has been reported. Our target was to explore the expression of pituitary tumor transforming gene (PTTG), matrix metalloproteinase-2(MMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) in ovarian serous tumor, investigating the function of PTTG1 in malignant transformation, invasiveness and metastasis of ovarian serous tumor.Methods:Experimental specimens were collected by the department of Pathology in the General Hospital in Tianjin Medical University from January 2004 to December 2008.The expression of PTTG1, MMP-2, VEGF and FGF-2 were examined by immunohistochemical technique in 30 ovarian serous cystadenoma and 21 ovarian borderline serous cystadenoma as well as 30 ovarian serous cystadenocarcinoma including primary and metastatic sites. The data was analyzed by SPSS software.Results:1. There was a significant difference of the expression of PTTG1 among each group of ovarian serous tumor (P<0.01), PTTG1 expression in the ovarian borderline serous cystadenoma was higher than ovarian serous cystadenoma (P<0.01),in the ovarian serous cystadenocarcinoma primary site was higher than ovarian borderline serous cystadenoma (P<0.01), in the ovarian serous cystadenocarcinoma metastatic site was higher than primary site (P<0.05);2. The expression of MMP-2 in ovarian serous cystadenocarcinoma primary site and ovarian borderline serous cystadenoma was higher than ovarian serous cystadenoma (P<0.01),in the ovarian serous cystadenocarcinoma metastatic site was higher than primary site (P<0.05);3. VEGF expression in the ovarian serous cystadenocarcinoma primary site and ovarian borderline serous cystadenoma was higher than ovarian serous cystadenoma (P<0.05),there was no statistic difference of the VEGF expression between the primary site and metastatic site of ovarian serous cystadenocarcinoma (P>0.05)4. There was a significant difference of the expression of FGF-2 among each group of ovarian serous tumor (P<0.01), FGF-2 expression in the ovarian borderline serous cystadenoma was higher than ovarian serous cystadenoma (P<0.01),in the ovarian serous cystadenocarcinoma primary site was higher than ovarian borderline serous cystadenoma (P<0.01), there was no statistic difference of the FGF-2 expression between the primary site and metastatic site of ovarian serous cystadenocarcinoma (P >0.05);5. In ovarian serous tumor, there were remarkably positive correlation among the expression of PTTG1, MMP-2,VEGF and FGF-2.Conclusions:The expression degree of PTTG1 proteins was corresponding with the tumor malignant degree, which may be cause the malignant transformation. The expression degree of PTTG1 proteins was consort with the capability of tumor invasiveness and metastasis, which may be contribute to the tumor invasiveness and metastasis.PTTGl probably participate the malignant transformation and also play an important role in angiogenesis, invasiveness and metastasis of ovarian serous tumor by up-regulate MMP-2, VEGF and FGF-2.Artificially inhibit or interfere the expression of PTTG1 in ovarian serous tumor would better than just control only one protein expression of MMP-2, VEGF and FGF-2. This study may provide a new therapeutic strategy for the ovarian serous tumor.