The Study On The Expression Of Keratinocyte Growth Factor (KGF) In OLK And OSCC

Objective:Oral leukoplakia (OLK) is a damage mostly with white colour in the oral mucosa and without other characters that can be defined. It is general precancer. And some cases can turn into cancer. The epithelial hyperplasia of the oral mucous membrane is the essential feature of the precancerous lesion which is the transform stage from normal condition to malignant cancer. Researches show that 4%-40% out of the OLK cases would become cancers. The risk of OLK cases that turn into cancer is 50-100 times more than normal persons. It is a process of many stages from OLK to OSCC affected by a series of factors. Oral squamous cell carcinoma (OSCC) is major carcinoma in oral tumor. Some results of recent experiments show that KGF may play a role in the process of tumor's occurance, development and metastasis. The aim of this study is to detect the different expression of KGF in the tissue of normal oral mucosa, OLK and OSCC at the level of mRNA and protein by ISH and Immunohistochemistry. And it is to be discussed about the function and mechanism of KGF in the transformation from oral precancer to cancer and OSCC metastasis. Methods:1. The three different types of fibroblasts of normal oral mucosa, oral leukoplakia and OSCC were cultured in vitro. The proliferative activity of the three different types of fibroblast is detected by MTT technique. And IH technique is adopted to detect the expression of KGF, vimentin and cytokeratin.2. The study group consisted of 34 cases, included 10 cases of OLK, and 11 cases of OSCC, and 13 cases with normal oral mucosa. These specimens were all defined...