A Study On The Expression Of Tumor Specific Antigen MAGE, BAGE, GAGE In Ovarian Cancer Tissue And Ovarian Cancer Cell Lines

Objective To observe the expression of MAGE, BAGE and GAGE gene in ovarian cancer tissue and cell line ; To investigate the effect of 5-aza-2 ' -deoxycytidine in proliferation of ovarian cancer cell line and expression of tumor specific antigen MAGE BAGE GAGE.Methods The expression levels of MAGE-1, MAGE-3, GAGE-1/2 and BAGE was detected by reverse transcription polymerase chain reaction(PT-PCR) in ovarian cancer cell line SKOV3, A2780,COC1 ,and 10 cases of normal ovarian tissues, 20 cases of benign epithelial tumor, 47 cases of malignant epithelial tumor ; Ovarian cancer cell were treated with 5-aza-2' -deoxycytidine(DAC) in co-culture, the growth curves were observed; the cell cycle was detected by flow cytometry(FCM);the gene expression pattern before and after drug treatment was measured by RT-PCR.Results Normal ovarian tissue does not express members of the gene families studied. In 3/20 benign tumor tissue specimens expressed MAGE-1.The ovarian cancer tissue samples expressed MAGE-1,MAGE-3,GAGE-1/2 and BAGE in 51.1% (25/47), 34% (16/47) , 25.5% (12/47) and 14.9% (7/47) .A total of 2/7 metastatic lesions from ovarian carcinoma expressed MAGE-1 and one expressed BAGE. There is preferential expression of the...