A Study On The Expression Of Tumor Specific Antigen MAGE, BAGE, GAGE In Endometrial Carcinoma And Cell Line

Objective: The aim of this study was to observe the expression of MAGE, BAGE , GAGE genes in endometrial carcinoma , normal endometrium and endometrial carcinoma cell line (KLE), and to evaluate the possibility of using these genes as molecular markers and as the targets of specific immunotherapy for endometrial carcinoma. The other aim of this study was to investigate the effect of 5-aza-2'-deoxycytidine(DAC)in proliferation of endometrial carcinoma cell line and expression of tumor specific antigen MAGE, BAGE, GAGE .Materials and Methods: Samaples of 35 endometrial carcinoma, 15 normal endometrial tissues and endometrial carcinoma cell line were examined with reverse-transcription polymerase chain reaction (RT-PCR) for the expression of MAGE-1, MAGE-3, BAGE and GAGE1-2. Endometrial carcinoma cell were treated with 5-aza-2'-deoxycytidine (DAC) in co-culture, the growth curves were observed; the genes expression pattern before and after drug treatment was measured by RT-PCR.Results: In 35 endometrial carcinoma of samples, MAGE-1, MAGE-3, BAGE and GAGE1 -2 mRNAs were expressed in 46% (16/35), 49% (17/35), 20% (7/35) and 26% (9/35) , respectively. At least one transcript was detected in 77%. While no expression was observed in 15 normal endometrial tissues. There was no significant...