Investigation Of Somatostatin Analogue Octreotide's Effect On The Experimental Choroidal Neovascularization

Objective: To observe the effect of somatostatin analogue octreotide on b-FGF,TGF-pin the experimental choroidal neovascularization (CNV) of mice, investigating octreotide as an inhibitor on the formation of CNV. Methods: Diode laser was applied to the eyes in C57BL/6 mice to induce choroidal neovascularization(CNV). We chose post-photocoagulation 7d, 14d, 21d and 28d as observed time point. In all 135 mice, 3 mice as normal control, 3 in one observed point as diseased control, 120 mice treated with octreotide were divided into 2 groups: group P0 was treated as soon as finished laser photocoagulation, group P7 was treated 7 days after photocoagulation. Each groups had three sub-groups: simply treated with subcutaneous injection of octreotide, with retrobulbar injection, or two methods combined. The dose of octreotide was 50μg/kg, qd×l4d. At each observed point, mice were sacrificed, the eyes were removed, Reverse transcription poly chain reaction (RT-PCR) was used to detect the changes of b-FGF or TGF-β mRNA by different medications at different observed point, analysising the expression intensity and relative coefficient.All data were analyzed with SPSS 12.0, using t analysis. P<0.05, difference was significant; P<0.01, difference was extremely significant.Results: Fluorescein leakage and histopathological evidence of CNV were found one week after diode laser photocoagulation in C57BL/6J mice.RT-PCR showed that b-FGF or TGF-β mRNA were expressed in the sclera-choroid-retina complex.RT-PCR showed that the positive expression of b-FGF in CNVMs could not be inhibited by subcutaneous injection of octreotide in the early stage in group P0. In the late stage, it significantly decreased. In this group, retrobulbar injection or two methods combined were both effective. In group P7, subcutaneous injection of octreotide could not inhibit the expression of b-FGF in CNVMs, the other methods...