Detection Of Fusion Gene ETV6-NTRK3 In Adult Leukemia And Breast Cancers And Their Clinic Significances

Objective: To observe the therapeutic effect of two leukemia patients with fusion gene ETV6-NTRK3; To investigate the expression of fusion gene ETV6-NTRK3 in breast cancers; to search the relationship between it and the clinic characteristics; to approach the significance of the fusion gene in clinic diagnosis and evaluation of prognosis.Materials and Methods : (1) Following up two leukemia patients with fusion gene ETV6-NTRK3,observing the therapeutic effect.(2) Collecting the samples of formalin-fixed, paraffin-embedded tissue of breast cancers ( from Jinn central hospital , the Tumor hospital of Shandong Province and Huashan Hospital of Shanghai)of the past six years. Extracting total RNA with Trizol and amplifying specific fusion gene ETV6-NTRK3 by RT-PCR method;analysising the PCR products on 3% agarose gel electrophoresis.Meanwhile, observing the morphology of patients' pathology tissue slice; At last, making an analysis of the patients' clinic information to approach the significance of the fusion gene in clinic diagnosis and evaluation of prognosis.Results:(1)Analysis of two leukemia patients' clinical information with fusion gene ETV6-NTRK3: â‘  The types of the two patients with fusion gene ETV6-NTRK3 are all AML-M_2a.â‘¡ The two patients were applied to 2-3 standard dose DA or MA or HA therapy program and the results were NR. It hints that traditional chemical treatment is ineffective to...