Study On The Conformation Analysis, Activity Evaluation And The Operative Component Of AMP-2 To Decrease The Blood Sugar

This article use AMP-2( Amillaria mellea fruiting body is fragmented and then be hotted by water of 80℃ to extract carbohydrate) .And it invesgates the methods of isolated and purified AMP-2, these involve the main chemical component,decrease the blood sugar and the active site.On the basis of two majior chemical component flavone and carbohydrate of AMP-2, we can use methods of one of component.So I choose to use foramen magnum resin and Sephadex to separate the components.On the same time I can find the best parameter.The result of our experiments indicate the best paramete is water washing,0.32mL/min and one tube /12 min; And the best way for foramen magnum resin is the ratio of diameter and the length of the pivor bur, the best dentisy of solvent is 60% alcohol , best washing volum is 200mL.Using the substance from isolation to make a purity experiment and a experiment of decreasing blood sugar .The round polyacrylamide gel electrophoresis and the result of FPLC experiment indicate AMP-2-1, AMP-2-2 are both uniform component.While the result of foramen magnum resin indicats the water washing component and the 60% alcohol washing are not uniform.And Sephadex experiment indicates the AMP-2-1 and the water washing component are the same, and I call the 60% alcohol washing AMP-2-2'. As these we can see the Sephadex is the best way to separate AMP-2.The HPLC experiment make we know the molecular weight is that AMP-2-1 be 42 thousand and AMP-2-2 be 8 thousand. The main chemical component can be deduced by the infrared light spectrum and the ultraviolet spectrum . There are carbohydrate and some small aglycone . Polysaccharide content can be detected about 11.04% by the way of Phenol and sulfuric acid. Flavone content can be deteced 4.5% ultraviolet spectrophometer. In addition we also measure the water ratio of AMP-2 is 12.43%. And the GC indicate that the corformation monosaccharide of AMP-2-1 is Rha, Fuc, Ara, Xyl, Man, Glc, the corformation...