Improvement Of Rotavirus ELISA Test Method And Clinical Practice

OBJECTIVE: Rotavirus is one of the most important pathogenes in infant diarrhea. The test of rotavirus has important clinical value in the early diagnosis and distinguish diagnosis. If rotavirus is not early tested and antibiotic is misused, diarrhea would aggravate owing to the imbalance of bacterium in intestine, consequently disorders of water, electrolyte, and acid-base balance might happen, even to death. To test rotavirus of infant diarrhea by taking use of rotavirus kit is important and may direct rational use of medicine. So, to establish accurately and rapidly and simply method of testing rotavirus is of important significance to early certify pathogene and to control spread of rotavirus and to take effective treatment measure and to avoid misuse of antibiotic. We are going to improve the test conditions of Lanzhou's rotavirus ELISA kit in order to shorten time of testing rotavirus and meet clinical practice.METHODS:1 Improvement of Lanzhou's rotavirus ELISA kit1.1 Confirm the antigen-antibody reaction timeFirst, put 16 positive, 4 negative samples, 2 positive and 2 negative...