The Role Of ERK1/2 Signal Transduction Pathway In The Mossy Fiber Sprouting After Pilocarpine-Induced Seizure

Object:To investigate the role of ERK1/2 signal transduction pathway in the mossy fiber sprouting(MFS) after pilocarpine-induced seizures.Methods:To establish the C57BL/6 mice pilocarpine model of chronic epilepsy. Using immunohistochemistry to detect the spatiotemporal distribution pattern of p-ERK1/2,and using Neo-Timm staining to detect MFS in the inner molecular layer(IML) of dentate gyrus.Then applying SL327 (a specific inhibitor of MEK1/2) to interfere in the activation of ERK1/2,comparing the changes of MFS and analyzing the potential mechanisms.Results:1. Ethology observation:80%(28/35) of PILO-treated mice survived status epilepticus(SE),the mortality rate was 11.43%,and the spontaneous recurrent seizure(SRS) is 4.00±1.13 times/n.88%(31/35) of SL327-pretreated mice survived SE,the mortality rate was 2.86%,and the SRS was 2.07±0.80 times/n. The control mice didn't present any abnormal behaviors.