The Effect Of Saliva Miltiorrhiza Bugge On Human Periodontal Ligament Fibroblasts In Vitro

The key of periodontal tissue regeneration is the proliferation and differentiation of human periodontal ligament fibroblasts (HPDLFs) . The cells in pathological region are lack, so how can stimulate the proliferation and differentiation of HPDLFs is the key problem of research on periodontal tissue regeneration. The investigation in last years showed that Saliva Miltiorrhiza Bugge can improve the minimum-cycle, intervene the inflammation, adjust the synthesis of growth factors, and promote the tissue regeneration. The animal experiment suggested that Saliva Miltiorrhiza Bugge combined with biomembrane in GTR can promote the better periodontal tissue regeneration. The objective of this study is to investigate the effect of Saliva Miltiorrhiza Bugge on HPDLFs in vitro.HPDLFs were cultured with different concentration of Saliva Miltiorrhiza Bugge. The proliferation and differentiation of HPDLFs were observed. The result suggests that Saliva Miltiorrhiza Bugge can significantly stimulate the alkaline phosphatase activity, osteocaclin synthesis, and the proliferation of HPDLFs, and stimulate HPDLFs to become osteoblasts, when Saliva Miltiorrhiza Bugge's concentration is raised to 90mg/ml. The study indicates that Saliva Miltiorrhiza Bugge can efficaciously stimulate the proliferation and differentiation of HPDLFs in vitro, which may promote the periodontal tissue regeneration.