| OBJECTIVE:To investigate the effects of ecdysterone on survival of the rat's dorsal random pattern skin flap , and the possible mechanisms. METHODS:Twenty-four Sprague-Dawley rats were used in the study. There were sixteen male and eight female. The rats were equally divided into two groups of 12 each. A caudally based dorsal random pattern skin flap, measuring 2 cm ×8cm was symmetrically raised. In experiment group, at the time of 10 minutes before operation and the first to the fifth day after operation, 5mg/kg of ecdysterone was injected into rat's abdominal cavity. In the control group, the rats received the same amount of saline in the same way. Seven days after operation, the skin paddle survival of all the rats was measured by planimetry to get the survival area and proportion. Then the animals were sacrificed and the flap tissue samples were divided into two parts. One for SOD and MDA level measuring and the other for haematoxylin-eosinstaining and Ⅷ factor immuno—histological staining of the middle parts.
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