Expression Of GDNF, Fas And Caspase-3 Protein And Change Of Apoptosis In Neonatal Rats With White Matter Damage

Objective1 To set up the neonatal rat model of white matter damage (WMD); 2 To explore the mechanism of Fas, caspase-3 on the apoptosis in the WMD neonatal rats; 3 To observe the expression of GDNF protein and to explore the relationship between endogenous GDNF and apoptosis in the WMD neonatal rats. MethodsThe present study was conducted to employ 2nd-day SD rats. A total of 104 rats were randomly divided into experimental group (57) and control group (47).After hypoxia-ischemia 12 pups died in the experimental group and 2 pups died in the control group. The pups were perfused at 30min, 1h, 4h, 12h, 1d, 3d, 7d, 14d, 21d of recovery from hypoxic-ischemia. Immunohistochemi-cal technical was applied to investigate the changes of expression of GDNF,...