Study Of Decoction Of Atractylodoes And Astragali In Treating Ulcerative Colitis And Effects On Ornithine Decarboxylase

Backgrounds and aimsFunction of spleen in the theory of Traditional Chinese Medicine (TCM) is closed to gastrointestinal tract. Effect of herbs Replenishing Qi to invigorate the spleen may be relevant to effect of gastrointestinal tract protecting or recovery. To understand scientific connotation of spleen and deficiency of spleen systems, elucidating molecular pharmacological evidence for the clinical of Chinese herbs Replenishing Qi to invigorate the spleen is important.Before the study ,we have observed ornithine decarboxylase(ODC) and polymine expression in intestinal epithelial cell line (IEC-6) after treating with herbs Replenishing Qi to invigorate the spleen. We discovered some chemical composition in different site of white atractylodes rhizome, astragalus and liquorice have effects on cells proliferation and differentiation. Then on basis of traditional Broth of Atractylodoes and Astragali, we made up Decoction of Atractylodoes and Astragali preparation of three effective parts among mentioned chemical composition above. As a model of gastrointestinal mucosa damage, we desire to observe treatment effects of Decoction of Atractylodoes and Astragali in ulcerative colitis animal models. We expect to verify therapeutic effect of the drug on bowel mucosa damage and understand material basis of herbs Replenishing Qi to invigorate the spleen.Methods1. Study on making UC models in rat adopting TNBS clysmata.2. Screen effective dose of Decoction ofAtractylodoes and Astragali on UC models.3. Compare its pharmacodynamic action with Broth ofAtractylodoes and Astragali.4. Observe pathological alleosis procedure of UC and therapeutic action of Decoction of Atractylodoes and Astragal. In UC models observe ODC expression and change after drug treatment.5. Study on pharmacodynamic action of Decoction of Atractylodoes and Astragali in anti-inflammatory, relieving pain and inhibiting gastrointestinal mobility6. Study on acute toxicity of Broth of Atractylodoes and Astragali and Decoction of Atractylodoes and Astragali.Results1. UC models adopting TNBS clysmata is similar to human body in etiological factor and pathological change. But it is not stabilized. And much influencing factors can affect it. So preliminary experiment is necessary on same lot animal in same condition.2. Decoction ofAtractylodoes and Astragali is effective at dose of 0.4g/kg in treating UC.3. Curative effect ofDecoction ofAtractylodoes and Astragali surpasses traditional aqueous extract.4. UC rat were sacrificed in 10th days, 20th days and 30th days after treated with TNBS. Difference between stages in rat was obvious. Rat got worse after TNBS was used and pathological changes were the most serious in 7th days. Colon mucosa membrane in 10th days almost disappeared. Then weight of animal began to rise. Ulcer is common.20 days later, ailments got improve and new granulation tissue can be seen in microscopic. Therapeutic action of Decoction of Atractylodoes and Astragal was not apparent in acute stage and appeared in convalescence stage.5. ODC major localizes in mucous membrane. It seldom appears at normal condition. Only when damage occurs, translocation of ODC is a prerequisite for its regulation and function. At a fairly extent, increased polyamine levels depend on the tissue damaged extent. So ODC play an important role in tissue repair.6. Decoction ofAtractylodoes and Astragali has analgesia effect, can relieve the frequencyof acetic acid-induced stretching in mice. Its anti-inflammatory effect can lessen acute inflammation of ear. It can lighten granuloma weight induced by filter paper embed. It can step down the movement of defecate in mice. But it has no effect on diarrhea induced by folium sennae.7. Acute toxicity testing of Broth of Atractylodoes and Astragali and Decoction of Atractylodoes and Astragali are both very little. If s impossible to measure their LD50 by intragastric administration.Conclusion:Pharmacological research of Decoction of Atractylodoes and Astragali indicates herbs Replenishing Qi to invigorate the spleen can recovery bowel mucosa damage. ODC is one of its target protein.