Change Regularity Of Serum IgE In The Guiea Pig Death Of Anaphylactic Shock And The Study Of Mast Cell Degranulation

Objective Research the change regularity of serum IgE in the guiea pig death of anaphylactic shock that is cold-storaged in 4°C,and study mast cell degranulation with combined- staining,and investigate the role and meaning in the medicolegal expertise. So we can provide accurate and objective diadynamic criteria to the sudden erethistic death for the medicolegal expertise.Methods 64 guinea pigs are classified into the experimental group and the control group, with no purpose and each groups are classified into 0 hour after death group, 12 hour after death group, 24 hour after death group, 48 f hour after death group(Every group contains 8 guiea pigs).Establishing guinea pigs anaphylactic shock model,and making specimens of blood serum,and lung. (1)we tested the serum levels of IgE with ELISA. (2)we observe IgE in the lung of the guinea pig death of the anaphylactic shock with immunohistochemistry. (3)we study mast cell degranulation with BB-IG-MY combined-staining .Results (1)The concentrations of IgE of experimental group are higher than that of the control group.The serum level of IgE is no difference in the experimental groups 48 hours after death. (2)The result of immunohistochemistry dyeing indicate that there is some some pale yellow granula in the lung small vessle wall of the experimental groups.(3)Mast cell have released their granules.Conclusion Measuring the serum level of IgE can provide objective diadynamic criteria to diagnose anaphylactic shock for forensic experts. Mast cell have released their granules in the guiea pig death of anaphylactic shock.