The Expression Of The Tryptase And Chymase In The Traumatic Patients, AMI And The Human Sudden Erethistic Death

Objective:To complement Chinese healthy individuals' range of normal values of plasma tryptase levels through detecting 63 traumatic patients,21 acute myocardial infraction and 21 control group plasma tryptase levels,and to provide diagnoses supporting for anaphylaxis and anaphylactic shock;An investigation on the acute myocardial infraction and traumatic patients' plasma tryptase levels and plasma chymase activity,to find the relationship of the tryptase and the chymase in the plasma of traumatic patients;To explore the expression of tryptase and chymase in the pulmonary tissues of the human sudden erethistic death,trying to seek objective morphological reference support to diagnose sudden anaphylactic death for forensic medicine.Methods:Dividing experiment samples into five groups:the acute myocardial infarction(AMI) group,the fracture of lower limb group,the craniocerebral wounds group,the thoracic injuries group and the control group,there are 21 plasmas in every group.(1) Measuring plasma tryptase levels by the UniCAP 100 instrument(Pharmacia Diagnostics AB,Uppsala,Sweden).(2) Measuring plasma chymase activity by the enzyme-labelling measuring instrument(Thermo Electron Corporation,America).(3) tryptase and chymase were stained using fluoroimmunohistochemistry method,and the images of tryptase and chymase were analyzed by Image Analyze and Image-pro plus of OLYMPUSOLS.The positive cells were count.The levels of the tryptase and chymase was calculated.Result:(1) The level of tryptase in the control group is 2.97±1.05μg/L.The level of tryptase in the AMI is 1.59±0.59μg/L.The level of tryptase in the fracture of limb is3.87±1.50μg/L.The level of tryptase in the craniocerebral wounds is 3.51±0.85μg/L.The level of tryptase in the thoracic injuries is 3.79±1.50μg/L.Comparing with the arithmetic mean of plasma tryptase(3.06μg/L), the control group and the craniocerebral wound have no statistical significance(P>0.05).The AMI has statistical significance(P<0.05).The fracture of limb and the thoracic injuries have statistical significance(P<0.05).(2) the activity of chymase is related to the level of the tryptase(P<0.05).(3)There was a statistically significant difference(P<0.05) for the positive cell of the tryptase and chymase concentrations in the pulmonary tissues of the human between the group of anaphylactic shock(28.9±5.78) and the control groups(1.40±1.07).There was a statistically significant difference(P<0.05) for the tryptase and chymase concentrations in the pulmonary tissues of the human between the group of anaphylactic shock(94.84±2.74) and the control groups(0.87±0.54). Conclusion(1)AMI can influence the level of tryptase.The craniocerebral wounds don't influence the level of tryptase,the fracture of limb and the thoracic injuries cause the level of tryptase increased lightly.(2)The activity of chymase is related to the level of the tryptase in all groups.(3)The level of the tryptase and the activity of chymase were greatly enhanced in the pulmonary tissues of human with anaphylactic shock.