Experimental Study On Protective Effects Of Topical Hypothermia Irrigation On Secondary Spinal Cord Injury

Objective: This experiment investigated the possible mechanism on protective effects of topical hypothermia irrigation on the injured spinal cord of rabbits by studying the changes of the contents of H2O and Ca2+, histopathology, ultrastructure, apoptosis and neurofunction in spinal cord tissues after ASCI (Acute Spinal Cord Injury), and tried to look for a new way that alleviated the ACSI and improved the prognosis of the patients.Methods: Thirty-six New Zealand healthy rabbit were randomly divided into three groups: Group A: control group (n=12); Group B: SCI group (n=12); Group C: SCI with topical hypothermia irrigation group (n=12). Each rabbit in the three groups was anesthetized by injection of Sumianxin II (0.15ml/kg) from the ear-edge vein. Conventionally sterilizing and spreading aseptic towels, everyone was revealed to the spinous processes and neural scutes of T12~L2 and then cut off them, exposing the dura mater. Rabbits in Group A were sewed the wound up after exposing the dura mater for 20mins without trauma; Rabbits in Group B and C were made into ASCI models by improved Allen's weight-drop device, stroking the intensity...