| ObjectiveSpinal cord injury often leads to physical disability and psychical trauma. And, moreand more young adults have been suffering from spinal cord injury, which is a great burdenof society and families. Because the pharmacotherapies for spinal cord injury are limitedand the effects are not obvious at present, the therapies for spinal cord injury has alwaysbeen an emphasis of the clinical and basic research. Spinal cord injury includes primaryand secondary injury. Spinal cord ischemia-reperfusion injury (spinal cord ischemicreperfusion injury, SCII) is the major pathological mechanism in secondary injury. Duringthe secondary injury, the increased free radicals play an important role. It’s said thathydrogen could eliminate hydroxyl radical and peroxynitrite selectively in recent studies,and could produce a therapy effect on ischemia and anoxaemia. This study is about tomodel the SCII, inject hydrogen saline to rabbits enterocoelia, and then research theprotective effects of hydrogen saline on scavenging free radicals, adjusting the nerve cellapoptosis and suppressing the inflammatory response in SCII. This study provides not onlymore experimental evidences, but also new treatments and ideas for SCII and otherischemia/anoxaemia diseases.Methods1. Model SCII in rabbitsZIVIN method was adopted: used an aorta clamp to clip the abdominal aorta for35minutes, then opened the aorta clamp to induce SCII in rabbits.2. Experimental animals and groupingNew Zealand white rabbits(n=30). Animals were divided into3groups: GroupA(n=10): the rabbits were operated with laparotomy surgery and the abdominal aorta wasnot clipped. Group B(n=10): the rabbits were operated with laparotomy surgery andabdominal aorta was clipped for35minutes, then physiological saline was injected intoperitoneal cavity. Group C(n=10): the rabbits were operated with laparotomy surgery and abdominal aorta was clipped for35minutes, then hydrogen-rich saline was injected intoperitoneal cavity.3. Evaluate the motor function of hindlimbs in rabbitsThe motor function of the hindlimbs in different times was evulated through Tarlov5behavior scores.4. Histopathology evaluation in rabbit spinal cordRabbits were killed after72h, then the spina cord were taken out to make HE andTUNNEL.5. Evaluation of MDAcontent and CAT activityThe spinal cord were taken out when the rabbits were euthanized, then the MDAcontent and catalase activity were measured by lipid peroxidation MDA assay kit andcatalase assay kit.Results1. Talov5behavior scores resultsThe behavior scores of group Awere not descend. The scores of group B and group Cwere denscend distinctly after SCII. The scores of group C were better than that of group Bafter72h (p＜0.01).2. Results of histopathology of the spinal cord2.1HEThe boundary of grey and white matter in spinal cord tissue of group A was clear.Patterns of nerve cells and myelin sheath wrer integrate regularly. The boundary of greyand white matter in spinal cord tissue of group B was not very clear. Most nerve cells wereapoptosis after SCII. And endochylema was granular-vacuolar degeneration. The structureof the nerve cells were integrate in group C，only few neurons were vacuolar degeneration.2.2TUNNEL There were no apoptosis in groupA. In group B there were many inflammatory cells,and much apoptosis and karyopyknosis appeared. There were a small number ofinflammatory cells and a little apoptosis in spinal cord tissues in group C.3. Evulation of MDAcontent and catalase activityThe MDA contents in group B and group C were higher than that in group A. TheMDA contents in group C were lower remarkably than that in group B(P<0.05). Thecatalase activity in group Awas higher than that in group B and C. The catalase activity ingroup C was higher remarkably than that in group B(P<0.05).ConclusionsHydrogen-rich saline provides protective effects to SCII in rabbits by suppressingMDAand improving CAT activity, which are antioxidation and apoptosis resistance. |
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