Screening Of Adhesive Activity Of Probiotics And Preliminary Study On Models For Evaluating Probiotics Adherence Ability

A lot of factors in vivo can affect the growth of normal enterobacteria in gut and lead to its imbalance and diarrhea. Microecologic studies show that probiotics adhering to intestinal mucosal surface could evolve nature biologic barriers to decrease the occurrence rate of diseases caused by pathogens. And it could protect human gastrointestinal tract from pathogens invasion. The characteristics of adhesion of probiotics to the intestinal mucosal surface are a critical prerequisite for exerting beneficial effect to their host organisms. At present, there is few data about the comparition between the evaluaton models for probiotics. Therefore, we try to provide such data to establish a standard model, in our research.Methods: We used Gram-staining, isotope labeling, ELISA and adherence inhibition methold to evaluation the adherence ability of the bacteria of PEIFEIKANG products. By comparing of sensitivity, time, cost and convenience, the most satisfactory model was established. The adherence ability of probiotics was observed after S layer proteins of which were extracted by LcLi.Results: The Gram-staining results were in accordance with those of the other methods. And this model could be used as the ideal model for evaluation of adherence ability The adherence of probiotics was significantly impaired by the extraction of S layer proteins (P<0.01) We should try to avoid the influence of the chemical agent on S protenin..Conclusion: The Gram-staining model could be used as the ideal model for evaluation of adherence ability, with high sensibility and conveninence. The probiotics could inhibit the adherence and invasion of pathogens and attenuate their injuries to intestinal mucosal surface, because it was more sensible, stable, convenient and cost less tham other models.