Optimal Dosage Study Of Hepatic VX2 Tumor Chemotherapy By Intratumoral Injection Of Carboplatin Microspheres With Ultrasound Guided

Background:Primary hepatic carcinoma is a common malignancy of digestive system. It featured with high incidence, low surgical removal and high tumor recurrence. There are a number of methods for treating hepatoma, they all have advantages and disadvantages. One of the promising interventional methodology for hepatoma is ultrasound-guided percutaneous intratumoral chemotherapy. The therapeutic efficacy could be improved by changing the form of prepared drugs and the administration methods for intratumoral injection slow-realeased microspheres loading chemotherapeutics at the target site. Preliminary study showed carboplatin-loaded polylactic-co-glycolic acid microspheres (CBP-PLGA Ms) for treating rabbit VX2 liver tumor had better efficacy, less side effects over simple carboplatin by intratumoral injection. But the optimal dosage for this treatment need further study.Objective:To investigate the optimal dosage of CBP-PLGA Ms for treating rabbit VX2 liver tumor by intratumoral injection through exploring the inhibitory effects of different doses treatment groups of CBP-PLGA Ms on rabbit VX2 liver tumor. Then we compared the effectiveness of the optimal dosage CBP-PLGA Ms with ethanol by intratumoral injection on the rabbit VX2 liver tumor to provide theory foundament.Methods:1. CBP-PLGA Ms were prepared and their physical features were observed .CBP-PLGA-Ms were prepared by an acetone/mineral oil emulsion and solvent evaporation method.Then their shape, distribution and drug loading of the microspheres were tested.2. Model of liver VX2 tumor transplantation was established in rabbits.