Clinical Study On The Relationship Between Peripheral Blood CD4～+CD25～+ Regulatory T Cells Level And Renal Transplants Long-term Survival

Renal transplantation is an effective way to cure end-stage renal disease (ESRD). And short-term survival of renal tranplants rate (STSRTR) has significantly increased up to 90% because of the improvement of HLA-match and immunosupression .But the long-term survival rate of renal transplants (LTSRRT) still has no significantly increase and 10-year survival rate is just about 35%. That is because of chronic allograft nephropathy(CAN)and toxicity and adverse reactions of non-specificity immunosupression. Specific tolerance to allograft is the best way to reduce chronic rejection and side effects of immunosupression. There are evidences demonstrate that regulatory T cells(Tregs) are involved in the maintenance of tolerance to renal allografts and local suppressive factors are implicated in mechanism of tolerance.Among the CD4~+regulatory T cells basically two different subsets of Tregs can be differentiated by their distinct suppressive mechanisms. Naturally occurring CD4~+CD25~+Tregs exert their suppressive effects obviously via cell contact by membrane-bound molecules although the nature of these molecules is still elusive. The suppressive capacity of the second subset, Th3 and type 1 T regulatory (Tr1) cells, is contact independent and is based mainly on cytokines such as IL-10 and TGF-β1. It seems that these cells, in contrast to naturally occurring CD4~+CD25~+Tregs, represent altered states of differentiation rather than a unique cell lineage. However, the relationship between peripheral blood CD4~+CD25~+Tregs level and long-term survival is currently a matter of debate.To study relationship between peripheral blood CD4~+CD25~+Tregs level and renal transplants long-term survival and the role of IL-10 and TGF-β1.Methods:1. Detection CD4~+CD25~+Tregs level of PBL by means of FCM.