Effects Of Sirolimus-eluting Stents Implantation On The Expression Of The TNF-alpha

Objective: The purpose of this study was to determine the effects of Sirolimus-eluting stents on the expression of the TNF-alpha , the relationship between inflammation and in-stent restenosis and the mechanism of Sirolimus-eluting stents implantation for prevention of in-stent restenosis.Methods: Fourteen male miniature porcine (25 to 30 kg body weight) were randomized (seven animals per group) to receive either bare-metal stents (provided by dalian yinyi co.Ltd) or sirolimus-eluting stents (provided by microport medcial shanghai co.Ltd) in left anterior descendent (LAD) coronary artery. The guiding catheter was used as a reference to obtain a 1.1～1.2:1 stent-to-artery ratio compared with the baseline vessel diameter. The stented-coronary arteries were harvested at 4 weeks after stent implantation and Serial peripheral blood samples were obtained before stent implantation , at 1 and 4 weeks after the implantation. Reverse transcription polymerase chain reaction (RT-PCR) method was taken to detect the expression of TNF-alpha mRNA. The level of tumor necrosis factor-alpha (TNF-alpha) in serum were assessed by enzyme-linked immunosorbent assay (ELISA).Results:TNF-alpha serum levels in Sirolimus-eluting stents group did not differ before stent implantation , at 1 and 4 weeks after the implantation (420.0±32.5pg/ml,429.7±20.5pg/ml,438.9±34.1pg/ml...