Study On The Bone Marrow Cells In Myocardic Infartion

Objective: the mammalian heart was regarded as a postmitotic organ .thus ,the heart has a very limited regenerative capacityand responds to tissue injury by scar formation.Bone marrow cells(BMCs) , mainly including hematopoietic stem cells(HSC) and mesenchymal stem cells, (MSC) ,possess highly regenative potential. It can transdifferentiate into a wide variety of phenotypes ,such as cardiomyocyte and endothelial cell of th neovascular with the presence of a specific enviroment. The antigen CD34 is the marker on the surface of HSC. It also exists on the surface of mature vasoendothelium. CD133 (termed originally AC133),an early hematopoietic stem-cell marker, is an 120-Kda transmembrane polytide,expressed on HSC. It's not detectable on the surface of mature vasoendothelium. The 2 markers can characterize the BMCs.Method: in this study, we detect the expression of CD34 ,CD133 and TGF-βin the autospised hearts of myocardiac infartion and normal hearts of 15 cases respectively, aming to analyze the homing of BMCs after infartion.Results: in the normal group, the expression of CD34 seems like a thin-line around the heart tissue. In the infarted area and border zone, the cells of CD34 positive form vascular. CD133 was not detectable in the normal heart tissue ,but expressed in the infarted area and border zone. some of the CD133-positive cells form neovascular. TGF-βis also not detectable in the normal group. In the infarted area and border zone of the heart tissue, TGF-βis detectable. It was found in the plasma of the endothelium of the neovascular.