Effects Of Vitamin E Succinate On Cisplatin-induced Hepatocyte Cytotoxicity And Antiproliferative Activites For Tumar Cells In Vitro Cells

Introduction: Vitamin E Succinate(VES) is the most active derivatives of Vitamin E. Early reports show that VES effectively inhibits the growth of several cancer cells in vivo and in vitro, such as human gastric adenocarcinoma cell, human breast cancer cell line, human prostate cancer cell, human colon cancer cell line and so on. Although the exact mechanisms remain largely unclear, previous studies suggeste that VES arreste the cycle of cancer cells at G1, promotes the proliferation, inhibites DNA synthesis and induces apoptosis. Some reports show that after VES triggeres Fas singaling system, the combining of FADD and caspase-8 activates, caspase linked responses, and there by the apoptosis singaling system was started. It is noted that VES does not inhibit proliferating of normal cells, but chemotherapeutic drugs has not this selectivity. Cisplatin(CP), a commonly used anticarcinomo drug, is the chief chemotherapeutic agent in clinical treatment of cancers, including the head, neck, testis and ovary. Unfortunately, it has been found that there are many side effects for this medicine, such as bone marrow suppression, hepatotoxicity and nephrotoxicity, and these side effects limite the dose of CP and its curative effect. At present, there is much more to ameliorate toxicity. In this study, we investigated the effects of VES on CP-induced hepatocyte cytotoxicity and the plausible mechanisms of combination of VES and CP.Method: Human and rat hepatocytes isolated were inoculated to 96-well collagen-coated plate. After cell attachment, different concentration solution of CP, VES and CP+VES were added to each well and incubation...