In Vitro Study Of The Relationship Between Neurotrophin-3 And Perineural Invasion In Salivary Adenoid Cystic Carcinoma

Adenoid cystic carcinoma (ACC) has a characteristic of perineural invasion. Neurotrophin-3 (NT-3), a member of neurotrophin family, can regulate nerve development, growth and regeneration of both peripheral nervous system and central nervous system. Our team's early research showed enhanced expression of TrkC and NT-3 in salivary ACC. Further in-vitro studies on secretion of NT-3 in ACC cells and the effects of NT-3 on invasion ability of ACC cells are necessary to gain better understanding of the relationship between NT-3 and perineural invasion in ACC. There are still no reports on secretion of NT-3 in ACC cells or its effects on the adhesion, migration and invasion ability of ACC cells.Objective: 1. To investigate the secretion of neurotrophin-3 in ACC cells in vitro; 2. To evaluate the effects of neurotrophin-3 on ACC cells' invasion ability in vitro; To provide evidence for understanding of the reasons for perineural invasion in ACC and for the controlling of ACC's perineural invasion.Methods: 1. Enzyme linked immunosorbent assay: Supernate of culture solution of ACC-M, ACC-2, M₃SP₄ and L929 were collected and NT-3 ELISA KIT was applied to measure the level of NT-3 in supernate. SNK-q test was used to evaluate the differences in means between groups. 2. Adhesion test in vitro: cell culture well was coated with Matrigel, and ACC-M cell line was...