Expression Of INOS In Transplanted Renal Tissue And The Effect Of AG/FK506 On Acute Allograft Rejection Following Renal Transplantation

Experiment 1AIM: To study and modify the techniques of orthotopic renal transplantation in rats, and to observe the acute allograft rejection of renal transplantation in recepted Wistar rats. METHODS: Orthotopic renal transplantation in 46 Wistar rats which had received SD renal grafts was performed by using techniques improved in the vascular anastomoses and the procure of donor grafts. The thirty-six rats which survived more than 7 days after transplantation were randomly divided into the control group (n = 18) and the therapy group (n = 18), the therapy group was subcutaneously injected by FK506 0.1mg/kg·d daily for 1 week after operation. In the meantime, the acute allograft rejection of all renal transplanted rats were studied. RESULTS: In total 46 operations, 36 rats survived more than 7 days after transplantation, whereas 10 rats died. the operation time was 2~2.5h,including the warm ischemia time (5~10 min) , cold ischemia (15~30min),the time of vascular end-side anastomosis for inferior vena cava was 5.0~ 10.5 minutes, the...