Study On The Molecular Mechanism Of Rat Bladder Carcinogenesis Induced By Terephthalic Acid

Terephthalic acid (TPA), one of the most commonly produced chemicals in the world, had been extensively used for the synthesis of certain crystalline polyester resins, films, and fibers. With the increasing of the TPA production, the number of occupational workers has greatly increased. Though TPA is a non-genotoxic chemical, it induced bladder epithelial cell proliferation and ultimately bladder tumor as a result of the formation of urinary bladder calculi in chronic feeding studies. However, it is unclear whether TPA plays a role of promoter in rat bladder carcinogenesis. The molecular mechanism of rat bladder carcinogenesis promoted or induced by chronic mechanical stimulation of bladder calculi remains unclear. In this study, we try to explore the molecular mechanisms of rat bladder carcinogenesis by establishing two-stage and middle-term TPA carcinogenic animal models.Part I. Study on the molecular mechanism of rat bladder carcinogenesis promoted by terephthalic acidObjective: The tumor-promoting effect of terephthalic acid and the molecular mechanism of rat bladder carcinogenesis were studied by establishing a two-stage rat carcinogenic model.Methods: A total of 190 male Wistar rats at age of 6 weeks were randomly divided into 10 groups. Rats in groups 1-5 were treated with MNU (20 mg/kg b.w. ip) and those in 6-10 groups with citrate buffer...