Constructing Of Cloned Esophageal Squamous Cancer Cell Strain With Hypoxia-Inducible Factor 1α Gene Silencing And Initial Study On The Role Of HIF-1α Gene In Esophageal Squamous Cancer Cell

Objective:Esophageal Squamous Cancer is one of the common cancers in digestive system with high malignancy, which has poor prognosis and many difficult in therapy. The mechanisms of its pathogenesis and progression are still not clarified fully. It is important to understand deeply its mechanism to help early dignosis, find more effective methods of treatment and evaluate prognosis more accuratly. We design the study to constructe cloned esophageal squamous cancer cell strain with hypoxia-inducible factor 1 α (HIF-1α) gene silencing by RNA interference and to determine the role of HIF-1 α gene in esophageal squamous cancer cells, in order to make a basis for more deeply study in esophageal squamous cancer and search for more effective therapeutics.Methods:1. We observed the specific inhibition of Green Fluorescent...