Effect Of Silencing HIF-1? By RNA Interference On Expression Of Vascular Endothelial Growth Factor In Human Glioma Cell Line A172 Under Hypoxia

Objective Gliomas are among the most difficult diseases to be treated in neurosurgery,owing to the high invasion,high metastasis,and high mortality of these malignant tumors[1].Although glioma suppression is the goal for all neurosurgeons and academics in the field,current research and clinical feedback indicate that we have a long way to go before we reach this goal.It now appears that the main element of patient survival is the World Health Organization classification of the tumor,and whether the maximum extent of resection has been achieved[2-6].The existence of the blood–brain barrier increases the difficulty of treatment.Numerous studies have shown that vascular endothelial growth factor(VEGF)can promote tumor growth.Hypoxia-inducible factor 1-alpha(HIF-1?)not only promotes tumor growth,but also regulates VEGF expression and promotes the growth of the blood supply inside the tumor[7].Here,we aimed to reduce gene expression of HIF-1? gene through silencing expression of the HIF and VEGF genes in tumor cells,in order to limit the growth of glioma.Methods Chemical hypoxia induced by Co Cl2 was used to simulate the tumor hypoxia environment.Real-time quantitative reverse-transcription PCR(real-time q RT-PCR),immunofluorescence,and Western blotting were used to compare the expression of VEGF protein and m RNA in A172 cells before and after transfection to silence the HIF-1? gene.Results After successful transfection,the expression of HIF-1? protein and m RNA in hypoxia for 6,12,18,and 24 h was significantly lower than before transfection,and the expression of VEGF also decreased over 24 h.Conclusions After silencing of the expression of HIF-1? gene,the expression of VEGF in A172 cells was also decreased after a period of time.