Expression And Correlation Of C-fos, EGF And EGFR In Human Testis During The Development

Objectives: ①To observe the distribution of the epidermal growth factor(EGF) and epidermal growth factor receptor(EGFR) in human testis during embryonic development. ②To observe the distribution of the c-fos in Human Testis During embryonic Development.③To observe the regularity of expression and localization of c-fos, EGF and EGFR in human testis, infer their action and interface in human testis tissue during the development.④in order to offer theoretical warranty for physiology of male reproduction.Methods ①Collect the 32 cases of normal testis from human fetuses (estimated gestational ages of 12 weeks to 38 weeks) were used. These samples were divided into nine groups according to gestational ages; ②Hematoxylin-Eosin(HE) staining; ③ Immunohistochemical ABC method was used.④The computer Image Analyser.⑤disposed by SPSS statistical software.Results: ①Under TEM the tissue of testes of 12 weeks is immature, volume is small. The number of cellular organ in cytoplasm is less, volume is small. Fatty substance vacuole can be seen by chance. The volume of cells is more large opposite of 20 weeks' testes tissue, the number of cellular organ in cytoplasm is more, mitochondria, endoplasmic reticulum(ER) fatty substance vacuole can be seen. ②HE staining: The tissue of testes of 12 weeks have not differentiation, as cord. lobules of testis may be seen indistinctly in fetus tissue of testes of 14 weeks, but seminiferous tubule can't difference. seminiferous tubule discernable after 16 weeks, but without lumens. ③immunohistochemistry: The expressions of EGF and EGFR were observed in human fetal testis. The immunoreactive cells were observed in 12 weeks tistis. The immunoreactive cells of 16 weeks and 20 weeks add gradually. The...