| Objective To establish the model of nonalcoholic fatty liver disease and investigate the effects of meltatonin on the level of serum transaminase , lipid, SOD, GSH-PX , MDA, hepatic morphology and the expression of CYP2E1 and HO-1 in rats with nonalcoholic fatty liver disease.Methods Fifty male Wistar rats were divided into five groups with ten for each randomly. The melatonin in low does, moderate does, high does group and model group were fed with high fat diet while the normal control group with common diet for 12 weeks. Melatonin ( contain≤1 % dehydrated alcohol) was intraperitoneally injected to the rats in various melatonin groups ( 2.5mg/kg, 5mg/kg and 10mg/kg ,respectively ) and equal does of saline ( contain 1 % dehydrated alcohol) to control and model group once a day. After 12 weeks experiment ,all rats were sacrificed. Histopathological changes in liver ,liver humid weight and liver index were observed.The level of AST and ALT in serum, TG, TC, SOD,MDA and GSH-PX in serum and liver tissue homogenates were measured by biochemical methods.The expression of CYP2E1 and HO-1 in liver was observed by immunohistochemical method.Results the NAFL/NASH rat model was successfully established with high fat diet for twelve weeks. Compared with control group, the model group developed moderate -serious steatosis and focus infiltration of inflammatory cells and punctiform necrosis in hepatic lobules,with significant increase on liver humid weight,liver index , level of serum AST,TC,MDA (P<0.01) , serum ALT and TC, TG and MDA in liver tissue homogenates (p<0.05),simultaneously with decrease on serum and liver tissue homogenate SOD , GSH-PX (p<0.05).The expression of CYP2E1 and HO-1 in liver was also increased .The effect of moderate,high does of MT on rat nonalcoholic fatty liver...
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