The Prelimitary Research Of Plasm Metabolite In Liver Transplant Patients Using Metabonimic Method

Liver transplantation is effective for treating patient with liver disordes in the late stage which behave abnormal metabolite condition and the normal liver function and metabolite condition will gradually recover after donor liver replacing ill-liver, in which represent different metabolite character. The state of plasm metabolite of liver transplantation could be analysed by using metabonimic method and different metabolite changes can be used to estimate clinical effect.The result of analysis show characteristic [~1H] nuclure magnetic resonance (NMR) fingerprint of plasm metabolite and specifical distributed score scattering plot of principal components analysis (PCA) which relate to liver function and metabolite condition reestablished gradually at different times before and late liver transplantation. On the orther hand , the blood glucose and lactate level increase and lipid content decrease at first day and blood glucose maintain still high level, lactate reduces, and lipid keep up to be raised at one week after transplant operation. During one to three month glucose recover to normal leveal and lipid still keep on high content and lactate level increase once more, which suggest abnormal liver perfusion and function insufficiency. At the same time choline increase markedly which abnormal change remind of possible residual tumor and likely cancer to be relapsed, which remain to be further investigated.In short, analysis of plasm metabolite in liver transplantation patients using metabonomics method can discover the characterisic fingerprint of plasm metabolite and different metabolite changes which could be used to estimate clinical effect of liver transplant patients.