Inhibition Mechanism Of Tea Polyphenols On Injury Of Human Umbilical Vein Endothelial Cells Induced By Oxidized Low Density Lipoproteins

Objectives Tea polyphenols is obtained from tea and is one of the activeingredients of tea. Abundant studies suggested that tea polyphenols is a kind of nature antioxidant and has a potent anti-oxidized property. Low density lipoprotein can be modified to oxidized low density lipoprotein(ox-LDL). It is generally thought that ox-LDL is the trigger of atherosclerosis because of its own cytoxicity and the formation of foam cells. Some studies data indicated that the injury of endothelial cells under the oxidative stress of ox-LDL was the important effect of its cytoxicity in vivo and in vitro. To study the mechanism of atherosclerosis promoted by ox-LDL, in the present study, we applied ox-LDL induced by copper ion to human umbilical vein endothelial cells(HUVEC), and followd by detection of cell proliferation, the changes of apoptotic associated proteins such as Bcl-2, Bax, caspase-3 as well as tubulin mRNA and proteins. These results can supply support for the measures of reducing the incidence of atherosclerosis and the farther abroad application of tea polyphenols.Methods LDL was oxidative modifided into ox-LDL by incubation with copperion in vitro. HUVEC were cultured under the condition of 37°C, 5%CO₂, saturation humidity. HUVEC lied in logarithm growth period were divided into control group and 3 experimental groups which were tea polyphenols, ox-LDL+tea polyphenols...