Yiqihuoxuejiedu Human Umbilical Vein Endothelial Cell Adhesion Induced By Oxidized Low Density Lipoprotein

Arteriosclerosis obliterans (ASO) is atherosclerotic material expanding and secondary thrombosis of lower extremity due to the narrow arteries, occlusive disease. Limb showed chills, numbness, pain, intermittent claudication, arterial pulse disappeared, Nutrition disorders, toe, foot or leg ulcers or gangrene occur. Serious decline in quality of life of patients, and even loss of limbs, serious and even life-threatening but also a great burden on society. With the improvement of living standards, changes in diet, and longevity, ASO incidence rates showed an upward trend, as serious harm to people's health, a common disease, and frequently-occurring, but also the numerous scholars of the important subjects question.In recent years, most scholars support the "theory of endothelial injury in response." That the major risk factors for this disease eventually intimal injury, atherosclerosis, is part of the initiating endothelial injury and endothelial dysfunction, oxidation of low density lipoprotein (OX-LDL) in causing endothelial dysfunction and atherosclerosis starts sclerosis (AS) play an important role.This study attempts to explore the qi, blood, detoxification method of Chinese medicine OX-LDL on human umbilical vein injury in endothelial cells (HUVEC) adhesion molecule during gene expression, transcriptional regulatory pathway and the role of monocyte adhesion mechanism for Combination therapy of Chinese medicine preparation arteriosclerosis obliterans of lower limbs to provide a theoretical basis for Chinese medicine treatment of lower extremity arterial occlusive disease pathogenesis and provide new ways of exploring a new research ideas and methods.1,OX-LDL-induced HUVEC and monocyte adhesion ratesObjective:Qi, activating blood circulation, detoxification method combination of different drugs on monocyte adhesion in rats.Methods:divided into 6 groups A, normal control group B, OX-LDL after injury HUVEC C. OX-LDL injury HUVEC+Qi, activating blood circulation, detoxification group D. OX-LDL injury in HUVEC+qi, blood group E. OX-LDL injury in HUVEC+blood, detoxification group F. OX-LDL injury in HUVEC+Qi, detoxification group. Extraction of human peripheral blood mononuclear cells and HUVEC adhesion in each group after 1h, BCA protein determination of each rate of monocyte adhesion. SPSS 16.0 statistical package for data analysis. Results:(1) OX-LDL significantly increased the human umbilical vein endothelial cells and mononuclear cell adhesion rate (P<0.01). (2) Qi, activating blood circulation, detoxify the triple combination of different drugs could reduce the HUVEC and mononuclear cell adhesion rate (P <0.01). (3) of different therapies and drug combinations group, mononuclear cell adhesion rates were significantly different:①Yiqihuoxue detoxification group the lowest rate of monocyte adhesion;②Yiqihuoxue the adhesion rate was higher value than not only Dosing of OX-LDL HUVEC injury group;③with detoxification therapies group low rate of monocyte adhesion.2, OX-LDL-induced HUVEC of ICAM-1, VCAM-1, NF-κB p65 protein expression Objective:Qi, activating blood circulation, detoxification method of different drug combinations on the OX-LDL after injury HUVEC of ICAM-1, VCAM-1, NF-κB p65 protein expression in the intervention.Methods:divided into 10 groups A, normal control group B, OX-LDL after injury HUVEC C. OX-LDL injury HUVEC+Qi, activating blood circulation, detoxification group D. OX-LDL injury in HUVEC+qi, blood group E. OX-LDL injury in HUVEC+blood, detoxification group F. OX-LDL injury in HUVEC+Qi, detoxification group. Protein SDS-polyacrylamide gel electrophoresis and Western blot assay after the intervention of Chinese medicine OX-LDL-induced HUVEC of ICAM-1, VCAM-1 and NF-κB p65 protein expression, SPSS 16.0 statistical package for data analysis.3, OX-LDL-induced HUVEC of ICAM-1, VCAM-1, NF-κB p65 in mRNA expression Objective:Qi, activating blood circulation, detoxification method of different drug combinations on the OX-LDL after injury HUVEC of ICAM-1, VCAM-1, NF-κB p65 in mRNA expression in rats.Methods:divided into 10 groups A, normal control group B, OX-LDL after injury HUVEC C. OX-LDL injury HUVEC+Qi, activating blood circulation, detoxification group D. OX-LDL injury in HUVEC+qi, blood group E. OX-LDL injury in HUVEC+blood, detoxification group F. OX-LDL injury in HUVEC+Qi, detoxification group. Real-time fluorescence quantitative PCR, after the Chinese intervention in OX-LDL-induced HUVEC of ICAM-1, VCAM-1 and NF-κB p65 of the mRNA. Using SPSS 16.0 statistical software measurement data with a mean±standard deviation between the measurement of multiple analysis of variance was used to compare data among the groups using LSD method, P＜0.05 considered statistically significant difference.Results:Qi, activating blood circulation, detoxification method can significantly reduce ICAM-1 and VCAM-1 in mRNA and protein expression levels, and o x-LDL group difference was significant (P<0.05, P<0.01).General Conclusion:Qi, activating blood circulation, detoxification method through the reduction in endothelial cell adhesion molecules; presumably by inhibiting NF-κβ,inhibition of monocyte-endothelial cell adhesion to vascular endothelial cells play a protective role in helping reduce or inhibit the formation of atherosclerosis.