Experimental Studies Of Vascular Allograft Of Seeded Rabbit Autologous Venous Endothelial Cells

Objective To study the feasibility of transplanting autologous venous endothelial cells to luminal surface of allogenic vein and increasing the long-term patency rate after transplantation.Methods Thirty health male rabbits were divided into two groups randomly,A and B. Autologous endothelial cells(group A)were harvested with 0.2% collagenase and cultivated in 60mm plastic culture plate to the second generation. The multiplied cells were lined in vitro onto the luminal surface of allogenic vein(group B)which disposal with freeze-drying for–700C and radiation with 0.2kGy. Incubated again for 9 days. The operations of venous grafting in situ were performed. The luminal surface of allogenic veins were observation with Light microscopy and Scanning electron microscope (SEM) ; Patency rate of allogenic vein were observation with Intravenous Digital subtraction angiography (IV,DSA) and color Doppler ultrasonosape.