Association Study Of HPV And Laryngeal Carcinoma In Crowd Of Beijing

The death rate of laryngeal carcinoma (LC) in China is the higest in the world. The association of human papillomavirus (HPV) infection with the development of LC has been extensively studied since the first report of the discovery of HPV in laryngeal lesions. Unfortunately, the results were controversial and inconclusive. There were marked variations in the observed HPV prevalence rate (3～100%). To elucidate the role of HPV in the development of LC, we employed polymerase chain reaction(PCR) based on nine pairs of primers, DNA sequencing and in situ hybridization (ISH)to screen the HPV infection in 123 LC tissues from Beijing District. Using PCR detection, 76.42% samples were HPV-L1 positive, 54.47% were -16E6 positive and 58.54% were -16E7 positive. The high incidence of HPV-L1 and together incidence of HPV-16E6, E7 genes suggest that L1 is fit for exame HPV infection and HPV might be integrated into tumor cells. The prevalence of HPV-18E6 infection was 42.28% , -18E7 infection was 48.78% , -31E7 infection was 10.57% , -33E7 infection was 8.13% , -45E7 infection was 7.32% and -52E7 infection was 12.20% . Our results support a role of HPV-16 infection in the pathogenesis of LC in Beijing area.