The Expression Of Livin In Gastric Cancer And It's Mechanism Of Resistance To Chemotherapy

Objective To study the expression of Livin in gastric carcinoma, and investigate it's role tumorigenesis, progression in gastric carcinoma and it's mechanism of resistance to chemotherapy,to supply a potent new target for therapy for gastric cancer. immunocytochemistyMethod (1) Detected the expression of Livin in SGC-7901 cells by immunocytochemisty(ICC). (2) Livin mRNA and protein expression in 30 cases of gastric cancers and sideward tissues of cancers and 6 cases these benign gastric tissues were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and western-blot. (3) Proliferation of SGC-7901 cells treated with 5-Fu was assessed by methyl thiazolyl tetrazolium (MTT) colorimetry. (4) The apoptotic cells and distribution of cell cycle were detected by flow cytometry. (5) Livin mRNA and protein expression in SGC-7901 cells before and after treated by 5-Fu were detected by RT-PCR and western-blot.Result Livin protein was expressed in nucleolus and cytokinesis of SGC-7901 cells.The positive percent of Livin expression was 66.67%(20 / 30 cases),which had significant difference (P<0.05). There were no...