Experiment Study Of Estrogen Receptor α And β Isoforms Expression In Ovary Of Mature Mice And In Vitro Cultured Follicles Of Immature Mice

Objective: To explore the roles of estrogen receptorα(ERα)and estrogen receptorβ(ERβ) Isoforms on follicular development and maturation of mice and to comprehend if there is difference between ERαand ERβexpression in follicles at all stages between in vivo maturation and in vitro culture. So as to learn more about the basic study of in vitro mature of immture oocyte by detecting ERαand ERβexpression in theca cells,interstitial cells and follicles of different stages of ovary in mature mice ,and by culturing preantral follicles of immature mice and detecting detecting ERαand ERβIsoforms expression in cutured follicles.Method: Detect distribution of ERαand ERβIsoforms on the ovaries of mature mice and follicles from cultured preantral follicles of immature mice by immunohistochemistry with rabbit-anti-mouse polyclonal antibody of ERαand ERβ, and quantitatively evaluate the staining intensity of follicles at different stages.Results: ovarian immunohistochemistry of mature mice showed that Positive ERαstaining was found in thecal cells and stromal cells and no positive ERβstaining in these two cells.ERαand ERβstaining were both negative in granular cells of primordial follicle,ERβstaining was positive in granular cells from primary up to preovulatory follicles,there was no...