Study On Pharmacodynamics Of Salvianolic Acid B On Myocardial Ischemia

Objective: To systemic study on Salvianolic acid B's medical effects on cardiovascular system, and to provide medical evidence for Sal.B's clinical use, we designed this experiment to observe how the Sal. B, ingredient abstracted from Salvia miltiorrhiza Bunge, affect myocardial ischemia, myocardial protection, and what is the effect on myocardial oxygen consumption after intravenous injected.Methods: To observe intravenous Sal.B's effect on myocardial ishcemia/reperfusion injury in SD rats, we built acute myocardial ischemia/reperfusion model by ligating left anterior descending branch of coronary arteries of rats. At the same time the ischemia is happening, saline, different dosages' Sal. B, Salvia miltiorrhiza Bunge solution, diltiazem solution were slowly intravenous injected, activity of serum creatine phosphokinase (CPK) and area of myocardial infarction (MI) were monitored under acute ischemia/reperfusion condition. To observe Sal.B's therapeutic effect on myocardial infarction model dogs, we built myocardial infarction model by ligating the left anterior descending branch of coronary artery of anaesthetized dogs. Saline, different dosages' Sal.B, Salvia miltiorrhiza Bunge solution and diltiazem solution were injected through femoral vein 10 minutes after ligation. The epicardial-electrocardiogram(N-ST, Σ ST), quantitative histology and serum lactatedehydrogenase(LDH) value were recorded. To observe Sal.B's effects on myocardial oxygen consumption and coronary blood flow of dogs, anaesthetized dogs were divided into 5 goups. Saline, different dosages' Sal.B, Salvia miltiorrhiza Bunge solution and diltiazem solution were intravenous injected. Before and 5, 15, 30, 60, 90, 120 minutes after injection, dogs' coronary...