| Objective:The aim of the Present study was to investigate the effee ts of Xin-ning tablet(XNT) on acute myocardial ischemia and hemo dynamics in anestheticzed dogs.Method:Myocardial infarction model was established by ligating the left coronary artery at the anterior descending branch in mongrels. It set up the model group, the positive medicine contrast groups(dil tiazemhydrochloride tablets and tongxinluo capsule)), and the group of XNT athigh, medium and low dosage. The influence of XNT myocardial infarction size was observed and the ST-segment elevati on in epicardial mapping (ECGG) was also determined, quantitative histology (N-BT s-taining) determination of infarct size,simultanteo usly determinate the coronary blood flow(CBF),myocardial oxygen c onsumption(MCO2),and the diversification of serum creatine kinase (CK), lactate dehydrogenase(LDH)and plasma endothelin (ET),T thr omboxane B2 (TXB2),6-keton-ProstaglandinF1。(6-Keto-PGF1a).and s tudy the XNT's influence of dogs'acute myocardial ischemia,my ocardial infarction and related indexes by digestive tract administrati on.The parameters of hemodynamics such as blood pressurt(BP), left ventricle systolie pressure (LVSP),CBF,coronary vascular resistance (CVR),MCO2.Results:XNT significantly diminished myocardial infarction size(?-ST), high-dose groups(HG)of XNT to produce a marked effect when dog s haved used the drugs for 30 minutes, and until to 180minutes.The ?-ST from (387.81±37.67) mv to (205.50±40.22) mv, decreased (46.59±11.65)%,Statistically significant difference(P<0.05～0.01); Middle-dose groups of XNT (MG)to produce a marked effect when dogs haved used the drugs for 45minutes, and until to 180minutes. The?-ST from (396.54±72.58) mv to (241.70±71.37) mv, decrea sed (38.42±15.21)%,statistically significant difference(P<0.05～0.01).XNT significantly reduced the range of myocardial ischemia(N-ST), HG to produce a marked effect when dogs haved used the dr ugs for 30 minutes, and until to 180minutes. The N-ST from (29.9 3±0.82) to (223.83±3.31), decreased (18.80±10.5) 5%,statistically s ignificant difference(P<0.05～0.01). MG to produce a marked effect when dogs haved used the drugs for 45minutes, and until to 180m inutes.The?-ST from (29.50±0.55) to (24.50±3.45), decreased (16. 88±12.05)%,statistically significant difference(P<0.05～0.01).XNT significantly reduced N-BT staining showed infarct area; HG infarct area account for (3.24±1.29)% and (7.59±2.78)%,Stati stically significant difference(P<0.01).; MG infarct area account for (4.03±2.25)% and (9.00±3.77)%,. statistically significant differen ce(P<0.01).XNT significantly increased the ischem c heart's CBF, the CB F of HG had a significant increase from 30minutes to 180minutes, Statistically significant difference(P<0.01); the CBF of MG had a si gnificant increase from 60minutes to 180minutes, statistically signifi cant difference(P<0.01).HG can reduced arterial oxygen content (AO2) at used the dru gs for 30,120,180minutes, and increased venous sinus oxygen c ontent (VO2)at used the drugs from 30minutes to 180minutes; redu ced MCO2 at used the drugs from 30minutes to 180minutes, statisti cally significant difference(P<0.05～0.01). MG can reduced AO2 at used the drugs for 30,60,180minutes, and increased VO2 at use d the drugs from 30minutes to 120minutes; reduced MC02 at used the drugs from 30minutes to 180minutes, statistically significant diff erence(P<0.05～0.01).XNT can also inhibit the releasing of serum lactate LDH,CK, HG significantly inhibit releasing of serum lactate LDH,CK from 120minutes to 180minutes,statistically significant difference(P<0.05～0.01); MG significantly inhibit releasing of serum lactate LDH,CK at 180minutes,statistically significant difference(P<0.05～0.01). HG and MG significantly inhibit releasing of serum plasma ET at used the drugs for 60,120minutes,statistically significant differe nce(P<0.05～0.01); HG and MG significantly inhibit releasing of pl asma lactate TXB2 at used the drugs for 15,30,120minutes,statisti cally significant difference(P<0.05～0.01); HG elevate plasma 6-Ket o-PGF1a from 15minutes to 60minutes,statistically significant differenc e(P<0.05～0.01); MG elevate plasma 6-Keto-PGF1a from 15minutes t o 60minutes,statistically significant difference(P<0.05～0.01); HG an d MG increased 6-Keto-PGF1a/TXB2 value from 15minutes to 120 minutes, statistically significant difference(P<0.05～0.01); Low-dose group(LG) increased 6-Keto-PGF1a/TXB2 value at used the drugs for 120minutes.The effect of XNT on systolic blood pressure (SBP),diastolic blood pressure(DBP),HR and electrocardiogram was not obvious.XNT significantly increased the ischem c heart's CBF, the CB F of HG had a significant increase from 30minutes to 180minutes, increased 30%,statistically significant difference(P<0.05～0.01);the C BF of MG had a significant increase from 60minutes to 90minutes, statistically significant difference(P<0.05); HG significantl decreased eoronary vaseular resistance (CVR) 30minutes to 180minutes,,statist ically significant difference(P<0.05～0.01)。MG significantl decreased eoronary vaseular resistance (CVR) 30minutes to 180minutes,statisti cally significant difference(P<0.05～0.01)。HG significantly increased left ventrieular work(LVW) from 45 minutes to 180minutes,statistically significant difference(P<0.05～0.0 1); MG significantly increased LVW from 45minutes to 180minutes,, statistically significant difference(P<0.05～0.01).The effect of XNT on left ventrieular systolic Pressure(LVSP). was not obvious HG significantly increased the maxium aseending r ate of left ventrieular pressure(dp/dtmax) from 45minutes to 90minute statistically significant difference(P<0.05～0.01);MG significantly in creased the maxium aseending dp/dtmax at used the drugs for 60min utes,statistically significant difference(P<0.05).HG significantly increased cardiac output(CO) from 30minutes t o 180minutes,statistically significant difference(P<0.05～0.01); MG si gnificantly increased CO from 30minutes to 90minutes,statistically si gnificant difference(P<0.05～0.01); HG significantly increased stroke volume (SV) from 30minutes to 180minutes,statistically significant difference(P<0.05～0.01); MG significantly increased SV from 30mi nutes to 90minutes, significant difference(P<0.05～0.01).HG significantly decreased total peripheral vaseular resistance(T PVR) from 30minutes to 180minutes,TPVR was (12162.59±1593.83) mmHg·min-1 before used the drugs,and reduced to (10297.96±2390.94) mmHg·min-1,statistically significant difference(P<0.05～0.01); MG sig nificantly decreased total peripheral vaseular resistance(TPVR) from 30minutes to 180minutes,TPVR was (11738.88±110.57)mmHg·min-1b efore used the drugs,and reduced to (10576.94±1486.27)mmHg·min-1, statistically significant difference(P<0.05-0.01).The effect of XNT on AO2 was not obvious, HG significantly increasedVO2 from 30minutes to 180minutes,statistically significant difference(P<0.05～0.01); MG significantly increased VO2 from 30mi nutes to 180minutes,statistically significant difference(P<0.05～0.01); LG significantly increased VO2 from 60minutes to 120minutes,statis tically significant difference(P<0.05～0.01).The effect of XNT on myocardial oxygen consumption index (MOCI) was not obvious. HG significantly increased MCO2 from 60minutes to 120minutes,statistically significant difference(P<0.05～0. 01),MG significantly increased MCO2 from 90minutes to 120minutes, statistically significant difference(P<0.05);HG significantly increased myocardial oxygen uptake rate (MURO) from 30minutes to 180min utes,statistically significant difference(P<0.05～0.01),MG significantly increased MURO from 30minutes to 180minutes,statistically signific ant difference(P<0.05～0.01),LG significantly increased MURO at us ed the drugs for60,90minutes,statistically significant difference(P<0. 05～0.01). Conclusions:XNT significantly decreased?-ST and N-ST, reduced N-BT sta ining showed infarct area,increased the ischemc heart's CBF,meanw hile, decreased AO2,increased VO2, thus reduced MCO2 It can also inhibit the releasing of serum LDH,CK activity and plasma ET,T XB2 release which were caused by myocardial ischemia and myocar dial infarction, increased plasma 6-Keto-PGF1a and 6-Keto-PGF1a/TX B2 value.The effect of XNT on HR,QRS,QT intervals and T-wave we re not obvious in anesthetized dogs, it increased DBP and the ische me heart's CBF, decreased CVR to Provide blood for ischemic he art, improvement LVW, increased dp/dtmax improve Myocardial activ ity ability, with positive regulatory role. meanwhile, increased CO a nd SV, thus BP had Raised,but TPVR was reduced; XNT significan tly increased VO2, decreased MCO2,MURO, improvement myocardi al oxygen metabolism,but the effect on AO2,MOCI were not obvio us in anesthetized dogs.
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